ISG15 Rabbit Polyclonal Antibody

CAT#: TA322671

Anti-ISG15 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 18 kDa. Positive control: Human brain tissue, Hela cell and mouse kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane 1: Human brain tissue lysate Lane 2: Hela cell lysate Lane 3: Mouse kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: ISG15 ubiquitin-like modifier
• Database Link:
  NP_005092
  Entrez Gene 298693 RatEntrez Gene 100038882 MouseEntrez Gene 9636 Human
  UniProt ID P05161
• Background: Interferon-stimulated 15 kDa protein (ISG15), also known as ubiquitin cross-reactive protein (UCRP), is a member of the ubiquitin-like protein family and functions in various biological pathways from pregnancy to innate immune responses. Expression of ISG15 is stimulated by cellular exposure to type 1 interferons a and β, in addition to infection with viruses such as influenza B. After exposure to type I interferons, both lymphocytes and monocytes, in addition to some fibroblasts and epithelial cells, release ISG15 into culture medium. ISG15 has been shown to function as a cytokine, stimulating interferon ? secretion by monocytes and macrophages, proliferation of natural killer cells, and chemotactic responses in neutrophils). ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by E1 (Ube1L), E2 (UbcH8) and E3 ligases via a multi-step process analogous to ubiquitination). ISG15 is removed from proteins by the ubiquitin processing protease Ubp43. ISG15-protein conjugation (ISGylation) is induced by type 1 interferons, and target proteins include the serine protease inhibitor Serpin 2A, PLC?1, ERK1/2, Jak1 and Stat1. Unlike ubiquitination, ISGylation does not target proteins for degradation, rather ISGylation increases Jak1 and Stat1 activity, enhancing the cellular response to interferons.
• Synonyms: G1P2; hUCRP; IFI15; IMD38; IP17; UCRP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: RIG-I-like receptor signaling pathway