ROCK2 Rabbit Polyclonal Antibody

CAT#: TA322700

Anti-ROCK2 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse brain tissue lysate Primary antibody: TA322700 (ROCK2 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322700 (ROCK2 Antibody) at dilution 1/130 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322700 (ROCK2 Antibody) at dilution 1/130, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse brain tissue lysate
  IHC: 50-200
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 300 amino acids of human Rho-associated, coiled-coil containing protein kinase 2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 161 kDa
• Gene Name: Rho associated coiled-coil containing protein kinase 2
• Database Link:
  NP_004841
  Entrez Gene 19878 MouseEntrez Gene 25537 RatEntrez Gene 9475 Human
  UniProt ID O75116
• Background: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis; smooth muscle contraction; the formation of actin stress fibers and focal adhesions; and the activation of the c-fos serum response element. This protein; which is an isozyme of ROCK1 is a target for the small GTPase Rho. Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction; actin cytoskeleton organization; stress fiber and focal adhesion formation; neurite retraction; cell adhesion and motility via phosphorylation of ADD1; BRCA2; CNN1; EZR; DPYSL2; EP300; MSN; MYL9/MLC2; NPM1; RDX; PPP1R12A and VIM. Phosphorylates SORL1 and IRF4.
• Synonyms: ROCK-II

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway