Nephrin (NPHS1) Rabbit Polyclonal Antibody

CAT#: TA322716

Anti-NPHS1 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse kidney tissue Primary antibody: TA322716 (NPHS1 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA322716 (NPHS1 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA322716 (NPHS1 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA322716 (NPHS1 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA322716 (NPHS1 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse kidney tissue
  IHC: 25-100
  Positive control: Human brain
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide peptide corresponding to a region derived from 1229-1241 amino acids of human nephrosis 1, congenital, Finnish type (nephrin)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 135 kDa
• Gene Name: NPHS1 nephrin
• Database Link:
  NP_004637
  Entrez Gene 54631 MouseEntrez Gene 64563 RatEntrez Gene 4868 Human
  UniProt ID O60500
• Background: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues; and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1; characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots; nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.
• Synonyms: CNF; nephrin; NPHN

Reference Data

• Protein Families: Druggable Genome, Transmembrane