IKB epsilon (NFKBIE) Rabbit Polyclonal Antibody

CAT#: TA322730

Anti-NFKBIE (Phospho-Ser22) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 40 kDa. Positive control: 293 cells treated with TNF lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: 293 cells untreated with TNF lysate Lane 2: 293 cells treated with TNF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat an ti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using NFKBIE (Phospho-Ser22) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-e.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: NFKB inhibitor epsilon
• Database Link:
  NP_004547
  Entrez Gene 18037 MouseEntrez Gene 4794 Human
  UniProt ID O00221
• Background: The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus.
• Synonyms: IKBE

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, B cell receptor signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway