BCR Rabbit Polyclonal Antibody

CAT#: TA322768

Anti-BCR Rabbit Polyclonal Antibody

Product Images

Predicted band size: 130; 160; 210 kDa. Positive control: HT-29, RAW264.7, Jurkat cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1-3: HT-29, RAW264.7, Jurkat cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using BCR antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCR, RhoGEF and GTPase activating protein
• Database Link:
  NP_004318
  Entrez Gene 110279 MouseEntrez Gene 613 Human
  UniProt ID P11274
• Background: A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ALL; BCR1; CML; D22S11; D22S662; PHL

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chronic myeloid leukemia, Pathways in cancer