STAT3 Rabbit Polyclonal Antibody

CAT#: TA322897

Anti-STAT3 (Phospho-Ser727) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 88 kDa. Positive control: Hela and HepG2 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: Untreated Hela cells lysate Lane 2: Hela cells treated with PMA lysate Lane 3: Untreated HepG2 cells lysate Lane 4: HepG2 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using STAT3 (Phospho-Ser727) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from Human STAT3.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88 kDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  NP_003141
  Entrez Gene 20848 MouseEntrez Gene 25125 RatEntrez Gene 6774 Human
  UniProt ID P40763
• Background: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
• Synonyms: ADMIO; APRF; HIES

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer