PKC mu (PRKD1) Rabbit Polyclonal Antibody

CAT#: TA322964

Anti-PRKCD (Phospho-Ser910) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 115 kDa. Positive control: EGF-treated 293 and PMA-treated HUVEC cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: EGF-treated 293 cells lysate Lane 2: Untreated 293 cells lysate Lane 3: PMA-treated HUVEC cells lysate Lane 3: Untreated HUVEC cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PRKD1 (Phospho-Ser910) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 910 (R-V-S(p)-I-L) derived from Human PKD/PKCµ.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 115 kDa
• Gene Name: protein kinase D1
• Database Link:
  NP_002733
  Entrez Gene 18760 MouseEntrez Gene 85421 RatEntrez Gene 5587 Human
  UniProt ID Q15139
• Background: PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion.
• Synonyms: PKC-MU; PKCM; PKD; PRKCM

Reference Data

• Protein Families: Druggable Genome, Protein Kinase