Phospholipase C gamma 1 (PLCG1) Rabbit Polyclonal Antibody

CAT#: TA322973

Anti-PLCG1 (phospho-Tyr771) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 155 kDa. Positive control: NIH/3T3 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: NIH/3T3 cells untreated with serum lysate Lane 2: NIH/3T3 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cell projection. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PLCG1 (phospho-Tyr771) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-?1.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 155 kDa
• Gene Name: phospholipase C gamma 1
• Database Link:
  NP_002651
  Entrez Gene 18803 MouseEntrez Gene 25738 RatEntrez Gene 5335 Human
  UniProt ID P19174
• Background: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection