FOXO1 Rabbit Polyclonal Antibody

CAT#: TA323073

Anti-FOXO1 (Phospho-Ser319) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 70 kDa. Positive control: NIH/3T3 cells untreated or treated with serum starvation lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: NIH/3T3 cells untreated with serum starvation lysate Lane 2: NIH/3T3 cells treated with serum starvation lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using FOXO1 (Phospho-Ser319) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using FOXO1 (Phospho-Ser319) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from Human FKHR/FOXO1A.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70 kDa
• Gene Name: forkhead box O1
• Database Link:
  NP_002006
  Entrez Gene 56458 MouseEntrez Gene 2308 Human
  UniProt ID Q12778
• Background: FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14
• Synonyms: FKH1; FKHR; FOXO1A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway, Pathways in cancer, Prostate cancer