EIF4B Rabbit Polyclonal Antibody

CAT#: TA323172

Anti-EIF4B Rabbit Polyclonal Antibody

Product Images

Predicted band size: 80 kDa. Positive control: 3T3 and HL60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1, 2: 3T3 and HL60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human lung carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human lung carcinoma tissue using EIF4B (Ab-422) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80 kDa
• Gene Name: eukaryotic translation initiation factor 4B
• Database Link:
  NP_001408
  Entrez Gene 1975 Human
  UniProt ID P23588
• Background: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
• Synonyms: EIF-4B; PRO1843

Reference Data

• Protein Pathways: mTOR signaling pathway