DNA Ligase IV (LIG4) Rabbit Polyclonal Antibody

CAT#: TA323263

Anti-LIG4 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 104 kDa. Positive control: K562, Jurkat and MCF7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 300 amino acids of human ligase IV, DNA, ATP-dependent
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104 kDa
• Gene Name: DNA ligase 4
• Database Link:
  NP_001091738
  Entrez Gene 290907 RatEntrez Gene 319583 MouseEntrez Gene 3981 Human
  UniProt ID P49917
• Background: Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.
• Synonyms: LIG4S

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining