NeuN (RBFOX3) Rabbit Polyclonal Antibody

CAT#: TA323300

Anti-RBFOX3 Rabbit Polyclonal Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse brain tissue lysate
  IHC: 50-200
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 88-102 amino acids of RNA binding protein, fox-1 homolog (C. elegans) 3
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: RNA binding protein, fox-1 homolog 3
• Database Link:
  NP_001076044
  Entrez Gene 52897 MouseEntrez Gene 287847 RatEntrez Gene 146713 Human
  UniProt ID A6NFN3
• Background: Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors); confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA; resulting in nonsense-mediated decay of the message; thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
• Synonyms: FOX-3; FOX3; HRNBP3; NEUN