GTP cyclohydrolase 1 (GCH1) Rabbit Polyclonal Antibody

CAT#: TA323417

Anti-GCH1 Rabbit Polyclonal Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 60 μg Lane: Human fetal liver tissue Primary antibody: TA323417 (GCH1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 15 seconds

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA323417 (GCH1 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA323417 (GCH1 Antibody) at dilution 1/15, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human fetal liver tissue
  IHC: 5-20
  Positive control: Human breast cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 51-64 amino acids of human GTP cyclohydrolase 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: GTP cyclohydrolase 1
• Database Link:
  NP_001019242
  Entrez Gene 14528 MouseEntrez Gene 29244 RatEntrez Gene 2643 Human
  UniProt ID P30793
• Background: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis; catalyzing the conversion of GTP into 7;8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however; not all variants give rise to a functional enzyme.
• Synonyms: DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways