ATP5PF Rabbit Polyclonal Antibody

CAT#: TA323495

Anti-ATP5J rabbit polyclonal antibody

Product Images

Predicted band size: 13 kDa. Positive control: Human fetal brain tissue and 293T cell, mouse intestinum tenue tissue and lovo cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane 1: Human fetal brain tissue lysate Lane 2: 293T cell lysate Lane 3: Mouse intestinum tenue tissue lysate Lane 4: Lovo cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer using ATP5J antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x 200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1000-2000, WB: 1:200-1000,IHC: 1:15-50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6
• Database Link:
  NP_001003696
  Entrez Gene 11957 MouseEntrez Gene 94271 RatEntrez Gene 522 Human
  UniProt ID P18859
• Background: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.
• Synonyms: ATP5; ATP5A; ATPM; CF6; F6

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease