ELK1 Rabbit Polyclonal Antibody

CAT#: TA323784

Anti-ELK1 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 45 kDa. Positive control: 293 and HT29 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1, 2: 293 and HT29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ELK1 (Ab-389) antibody at dilution 1/50. (Original magnification: ×200)

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  Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using ELK1 (Ab-389) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: ELK1, ETS transcription factor
• Database Link:
  NP_001107595
  Entrez Gene 13712 MouseEntrez Gene 314436 RatEntrez Gene 2002 Human
  UniProt ID P19419
• Background: Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
• Synonyms: ELK1; ELK1 protein; ETS-like gene 1; member of ETS oncogene family; OTTHUMP00000023224; OTTHUMP00000023225

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endometrial cancer, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Prion diseases