EIF4E Rabbit Polyclonal Antibody

CAT#: TA323840

Anti-EIF4E (Phospho-Ser209) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 25 kDa. Positive control: HT29 cells treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: HT29 cells untreated with Anisomycin lysate Lane 2: HT29 cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using EIF4E (Phospho-Ser209) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Cytoplasm. Positive control: MCF cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF cells using EIF4E (Phospho-Ser209) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  NP_001959
  Entrez Gene 1977 Human
  UniProt ID P06730
• Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Synonyms: AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway