AIF (AIFM1) Rabbit Polyclonal Antibody

CAT#: TA323845

Anti-AIFM1 Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 60 μg Lane: Jurkat cells Primary antibody: TA323845 (AIFM1 Antibody) at dilution 1/150 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323845 (AIFM1 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323845 (AIFM1 Antibody) at dilution 1/15, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323845 (AIFM1 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323845 (AIFM1 Antibody) at dilution 1/15, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Hela, A549, MCF7, HepG2, K562 cell lysates
  IHC: 100-300
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 250 amino acids of human apoptosis-inducing factor, mitochondrion-associated, 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67 kDa
• Gene Name: apoptosis inducing factor, mitochondria associated 1
• Database Link:
  NP_001124318
  Entrez Gene 26926 MouseEntrez Gene 83533 RatEntrez Gene 9131 Human
  UniProt ID O95831
• Background: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells; and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition; this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6; which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.?
• Synonyms: AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis