PKR (EIF2AK2) Rabbit Polyclonal Antibody

CAT#: TA323868

Anti-EIF2AK2 (Phospho-Thr446) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 62 kDa. Positive control: K562 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: Treated with the peptide Lane 2: K562 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using EIF2AK2 (Phospho-Thr446) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using EIF2AK2 (Phospho-Thr446) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: eukaryotic translation initiation factor 2 alpha kinase 2
• Database Link:
  NP_002750
  Entrez Gene 5610 Human
  UniProt ID P19525
• Background: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
• Synonyms: EIF2AK1; PKR; PPP1R83; PRKR

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors