14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: TA323869

Anti-YWHAZ (Phospho-Ser58) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 28 kDa. Positive control: Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: Hela cells untreated with UV lysate Lane 2: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Melanosome. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using YWHAZ (Phospho-Ser58) antibody at dilution 1/50. (Original magnification:×200)

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  Predicted cell location: Cytoplasm; Melanosome. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using YWHAZ (Phospho-Ser58) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  NP_663723
  Entrez Gene 22631 MouseEntrez Gene 25578 RatEntrez Gene 7534 Human
  UniProt ID P63104
• Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
• Synonyms: 14-3-3-zeta; HEL-S-3; HEL4; KCIP-1; YWHAD

Reference Data

• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection