OGA Rabbit Polyclonal Antibody

CAT#: TA323895

Anti-MGEA5 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse pancreas tissue Primary antibody: TA323895 (OGA Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA323895 (OGA Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA323895 (OGA Antibody) at dilution 1/15, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Mouse pancreas tissue
  IHC: 15-50
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm, Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 695-814 amino acids of human meningioma expressed antigen 5 (hyaluronidase)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 103 kDa
• Gene Name: meningioma expressed antigen 5 (hyaluronidase)
• Database Link:
  NP_001135906
  Entrez Gene 76055 MouseEntrez Gene 154968 RatEntrez Gene 10724 Human
  UniProt ID O60502
• Background: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT; which adds O-GlcNAc; and MGEA5; a glycosidase that removes O-GlcNAc modifications. Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. Shows highest expression in the brain; placenta and pancreas.
• Synonyms: MEA5; NCOAT; OGA