IDE Rabbit Polyclonal Antibody

CAT#: TA323993

Anti-IDE Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: K562 cells Primary antibody: TA323993 (IDE Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323993 (IDE Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323993 (IDE Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA323993 (IDE Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA323993 (IDE Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: K562 cells
  IHC: 10-50
  Positive control: Human colon cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a C terminal 300 amino acids of human insulin-degrading enzyme
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118 kDa
• Gene Name: insulin degrading enzyme
• Database Link:
  NP_001159418
  Entrez Gene 15925 MouseEntrez Gene 25700 RatEntrez Gene 3416 Human
  UniProt ID P14735
• Background: This gene encodes a zinc metallopeptidase that degrades intracellular insulin; and thereby terminates insulins activity; as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon; amylin; bradykinin; and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space; cell membrane; peroxisome; and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
• Synonyms: INSULYSIN

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease