VEGFA Rabbit Polyclonal Antibody

CAT#: TA324012

Anti-VEGFA Rabbit Polyclonal Antibody

Product Images

Predicted band size: 27 kDa. Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10seconds)

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  Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using VEGFA antibody at dilution 1/13, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:5000-240000, WB: 1:500-2000, IHC: 1:25-100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 23-36 amino acids of human vascular endothelial growth factor A
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27 kDa
• Gene Name: vascular endothelial growth factor A
• Database Link:
  NP_001165101
  Entrez Gene 22339 MouseEntrez Gene 83785 RatEntrez Gene 7422 Human
• Background: This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome; also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants; encoding either freely secreted or cell-associated isoforms; have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of; and in-frame with the first AUG; leading to additional isoforms.
• Synonyms: MGC70609; MVCD1; VEGF; VPF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway