FOXO4 Rabbit Polyclonal Antibody

CAT#: TA324038

Anti-FOXO4 (Phospho-Ser197) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 54 kDa. Positive control: 293 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: 293 cells untreated with serum lysate Lane 2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using FOXO4 (Phospho-Ser197) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using FOXO4 (Phospho-Ser197) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human FOXO4.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: forkhead box O4
• Database Link:
  NP_005929
  Entrez Gene 54601 MouseEntrez Gene 302415 RatEntrez Gene 4303 Human
  UniProt ID P98177
• Background: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
• Synonyms: AFX; AFX1; MLLT7

Reference Data

• Protein Families: Transcription Factors