Cortactin (CTTN) Rabbit Polyclonal Antibody

CAT#: TA324049

Anti-CTTN (Phospho-Tyr466) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 85 kDa. Positive control: Jurkat cells treated with H2O2 lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: Jurkat cells untreated with H2O2 lysate Lane 2: Jurkat cells treated with H2O2 lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1000
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: cortactin
• Database Link:
  NP_612632
  Entrez Gene 13043 MouseEntrez Gene 2017 Human
  UniProt ID Q14247
• Background: Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
• Synonyms: EMS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction