EGF Rabbit Polyclonal Antibody

CAT#: TA324064

Anti-EGF Rabbit Polyclonal Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: Human prostate tissue lysate Primary antibody: TA324064 (EGF Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA324064 (EGF Antibody) at dilution 1/140 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA324064 (EGF Antibody) at dilution 1/140, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human prostate tissue lysate
  IHC: 100-300
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 926-1026 amino acids of human epidermal growth factor
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 134 kDa
• Gene Name: epidermal growth factor
• Database Link:
  NP_001171601
  Entrez Gene 13645 MouseEntrez Gene 25313 RatEntrez Gene 1950 Human
  UniProt ID P01133
• Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth; proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor; epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants
• Synonyms: HOMG4; URG

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton