DOK1 Rabbit Polyclonal Antibody

CAT#: TA324071

Anti-DOK1 (phospho-Tyr362) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 62 kDa. Positive control: Jurkat cells treated with insulin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: Jurkat cells untreated with insulin lysate Lane 2: Jurkat cells treated with insulin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using DOK1 (phospho-Tyr362) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 362 (P-I-Y(p)-D-E) derived from Human p62Dok.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: docking protein 1
• Database Link:
  NP_001372
  Entrez Gene 13448 MouseEntrez Gene 312477 RatEntrez Gene 1796 Human
  UniProt ID Q99704
• Background: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
• Synonyms: P62DOK

Reference Data

• Protein Families: Druggable Genome