RhoGDI (ARHGDIA) Rabbit Polyclonal Antibody

CAT#: TA324107

Anti-ARHGDIA Rabbit Polyclonal Antibody

Product Images

Predicted band size: 23 kDa. Positive control: Jurkat, 293, A431, Hela and HL60 cell, mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane 1: Jurkat cell lysate Lane 2: 293 cel lysate Lane 3: A431 cell lysate Lane 4: Hela cell lysate Lane 5: HL60 cell lysate Lane 6: Mouse pancreas tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: Rho GDP dissociation inhibitor alpha
• Database Link:
  NP_001172006
  Entrez Gene 192662 MouseEntrez Gene 360678 RatEntrez Gene 396 Human
  UniProt ID P52565
• Background: Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization. The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI. Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA.
• Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway