STAT4 Rabbit Polyclonal Antibody

CAT#: TA324197

Anti-STAT4 (Phospho-Tyr693) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 86 kDa. Positive control: HepG2 cells treated with IL-4 lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: HepG2 cells treated with IL-4 lysate Lane 2: Untreated HepG2 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using STAT4 (Phospho-Tyr693) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 693 (K-G-Y(p)-V-P) derived from Human STAT4.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: signal transducer and activator of transcription 4
• Database Link:
  NP_003142
  Entrez Gene 20849 MouseEntrez Gene 367264 RatEntrez Gene 6775 Human
  UniProt ID Q14765
• Background: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.
• Synonyms: SLEB11

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway