ATF2 Rabbit Polyclonal Antibody

CAT#: TA324218

Anti-ATF2 (Phospho-Ser62 or 44) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 65-75 kDa. Positive control: MDA231 cells treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: MDA231 cells treated with UV lysate Lane 2: MDA231 cells untreated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

• 

  Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Ser62 or 44) antibody at dilution 1/50. (Original magnification:× 200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 62 or 44 (N-D-S(p)-V-I) derived from Human ATF2.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: activating transcription factor 2
• Database Link:
  NP_001871
  Entrez Gene 11909 MouseEntrez Gene 81647 RatEntrez Gene 1386 Human
  UniProt ID P15336
• Background: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
• Synonyms: CRE-BP1; CREB-2; CREB2; HB16; TREB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway