ATF2 Rabbit Polyclonal Antibody

CAT#: TA324219

Anti-ATF2 (Phospho-Thr69 or 51) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 65-75 kDa. Positive control: NIH/3T3 cells treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: NIH/3T3 cells untreated with Anisomycin lysate Lane 2: NIH/3T3 cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr69 or 51) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using ATF2 (Phospho-Thr69 or 51) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 69 or 51 (D-Q-T(p)-P-T) derived from Human ATF2.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: activating transcription factor 2
• Database Link:
  NP_001871
  Entrez Gene 11909 MouseEntrez Gene 81647 RatEntrez Gene 1386 Human
  UniProt ID P15336
• Background: Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
• Synonyms: CRE-BP1; CREB-2; CREB2; HB16; TREB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway