S6K1 (RPS6KB1) Rabbit Polyclonal Antibody

CAT#: TA324278

Anti-RPS6KB1 (Phospho-Ser424) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 70; 85 kDa. Positive control: MCF cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: MCF cells untreated with EGF lysate Lane 2: MCF cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

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  Predicted cell location: Cytoplasm, centrosomal and nuclear. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ribosomal protein S6 kinase B1
• Database Link:
  NP_003152
  Entrez Gene 72508 MouseEntrez Gene 83840 RatEntrez Gene 6198 Human
  UniProt ID P23443
• Background: Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
• Synonyms: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway