JNK1 (MAPK8) Rabbit Polyclonal Antibody

CAT#: TA324296

Anti-MAPK8 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: A172 K562 Jurkat cell lysates Primary antibody: TA324296 (MAPK8 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA324296 (MAPK8 Antibody) at dilution 1/95 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA324296 (MAPK8 Antibody) at dilution 1/95, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A172, K562, Jurkat cell lysates
  IHC: 100-300
  Positive control: Human thyroid cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a C terminal 300 amino acids of human mitogen-activated protein kinase 8
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48 kDa
• Gene Name: mitogen-activated protein kinase 8
• Database Link:
  NP_001265476
  Entrez Gene 26419 MouseEntrez Gene 116554 RatEntrez Gene 5599 Human
  UniProt ID P45983
• Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals; and are involved in a wide variety of cellular processes such as proliferation; differentiation; transcription regulation and development. This kinase is activated by various cell stimuli; and targets specific transcription factors; and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis; which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation; apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.?
• Synonyms: 2; JNK; JNK-46; JNK1; JNK1A2; JNK21B1; PRKM8; SAPK1; SAPK1c

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway