MCM7 Rabbit Polyclonal Antibody

CAT#: TA324302

Anti-MCM7 Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 50 μg Lane: Jurkat cells Primary antibody: TA324302 (MCM7 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA324302 (MCM7 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA324302 (MCM7 Antibody) at dilution 1/15, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA324302 (MCM7 Antibody) at dilution 1/15 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA324302 (MCM7 Antibody) at dilution 1/15, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat cells
  IHC: 15-50
  Positive control: Human ovarian cancer
  Predicted cell location: Cytoplasm, Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a C terminal 300 amino acids of human minichromosome maintenance complex component 7
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81 kDa
• Gene Name: minichromosome maintenance complex component 7
• Database Link:
  NP_001265524
  Entrez Gene 17220 MouseEntrez Gene 288532 RatEntrez Gene 4176 Human
  UniProt ID P33993
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2; 4 and 6 proteins possesses DNA helicase activity; and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase; CDK4; is found to associate with this protein; and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication