PKM2 (PKM) Rabbit Polyclonal Antibody [Clone ID: RB3697]

CAT#: TA324317

Pyruvate Kinase (PKM2) Antibody (C-term)

Product Images

Western blot analysis of anti-h PKM2-N491 Pab (Cat. #TA324317) in Ramos cell line lysates (35ug/lane). hPKM2-N491 (arrow) was detected using the purified Pab.

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  Pyruvate Kinase (PKM2) Antibody (C-term) (Cat. #TA324317) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were incubated with TA324317 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm.

Specifications

Product Data

• Clone Name: RB3697
• Applications: IF, IHC, WB
• Recommended Dilution: IF 1:200
  WB 1:1000
  WB 1:1000
  WB 1:2000
  IHC-P 1:50~1:100
• Reactivities: Human, Mouse, Rat, Monkey, Protozoa
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• Specificity: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57937 kDa
• Gene Name: pyruvate kinase, muscle
• Database Link:
  NP_001193725.1
  Entrez Gene 18746 MouseEntrez Gene 25630 RatEntrez Gene 697742 MonkeyEntrez Gene 5315 Human
  UniProt ID P14618
• Background: There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
• Synonyms: CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus