ALDH2 Mouse Monoclonal Antibody [Clone ID: 138CT22.3.8]

CAT#: TA324332

ALDH2 Antibody

Product Images

Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody (Cat. #TA324332). TA324332 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  ALDH2 Monoclonal Antibody (Cat. #TA324332) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of ALDH2 Antibody (Cat#TA324332) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

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  ALDH2 Monoclonal Antibody (Cat. #TA324332) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Specifications

Product Data

• Clone Name: 138CT22.3.8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF 1:10~50
  WB 1:1000
  IHC-P 1:50~100
  FC 1:10~50
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.
• Specificity: This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 56381 kDa
• Gene Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Database Link:
  NP_000681.2
  Entrez Gene 217 Human
  UniProt ID P05091
• Background: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.
• Synonyms: ALDH-E2; ALDHI; ALDM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation