ALDH1A1 Mouse Monoclonal Antibody [Clone ID: 152CT1.2.2]

CAT#: TA324334

ALDH1A1 Antibody

Product Images

ALDH1A1 Monoclonal Antibody (Cat. #TA324334) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).

• 

  ALDH1A1 Monoclonal Antibody (Cat. #TA324334) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

• 

  IF image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were incubated with TA324334 ALDH1A1 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.

• 

  Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#TA324334) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Clone Name: 152CT1.2.2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000
  IHC-P 1:50~100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.
• Specificity: This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54862 kDa
• Gene Name: aldehyde dehydrogenase 1 family member A1
• Database Link:
  NP_000680.2
  Entrez Gene 216 Human
  UniProt ID P00352
• Background: ALDH1A1 encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis.
• Synonyms: ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Metabolic pathways, Retinol metabolism