EIF4E Mouse Monoclonal Antibody [Clone ID: 163CT48.1.9]

CAT#: TA324335

Mouse monoclonal EIF4E Antibody

Product Images

EIF4E Antibody (Cat. #TA324335) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).

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  IF image of Hela cell stained with EIF4E Antibody (Cat#TA324335/SG101020AF).Hela cells were incubated with EIF4E primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). EIF4E immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Clone Name: 163CT48.1.9
• Applications: IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:100~500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25097 Da
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  NP_001959
  Entrez Gene 1977 Human
  UniProt ID P06730
• Synonyms: AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway