GFAP Mouse Monoclonal Antibody [Clone ID: 183CT3.1.5]

CAT#: TA324338

GFAP Antibody

Product Images

GFAP Antibody (Cat. #TA324338) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).

• 

  GFAP Monoclonal antibody (Cat. #TA324338) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody ( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

• 

  Confocal immunofluorescent analysis of GFAP Antibody (Cat#TA324338) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Clone Name: 183CT3.1.5
• Applications: IF, IHC, WB
• Recommended Dilution: IF 1:10~50
  WB 1:500~1000
  WB 1:4000
  IHC-P-Leica 1:1000
  IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
• Specificity: This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 49880 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  NP_001124491.1
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: ALXDRD

Reference Data

• Protein Families: ES Cell Differentiation/IPS