c Kit (KIT) Mouse Monoclonal Antibody [Clone ID: 566CT8.5.4]

CAT#: TA324353

Mouse monoclonal KIT Antibody

Product Images

KIT Antibody (Cat. #TA324353) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).

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  IF image of an SY5Y cell stained with TA324353 KIT antibody. SY5Y cells were incubated with TA324353 KIT primary antibody (1:500). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the KIT immunosignal to the membranes, especially the plasma membrane.

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  Immunofluorescent analysis of U251 cells, using KIT Antibody (Cat. #TA324353). TA324353 was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).

Specifications

Product Data

• Clone Name: 566CT8.5.4
• Applications: IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: This KIT Antibody is generated from mouses immunized with human KIT recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 109865 Da
• Gene Name: KIT proto-oncogene receptor tyrosine kinase
• Database Link:
  NP_000213
  Entrez Gene 3815 Human
  UniProt ID P10721
• Synonyms: C-Kit; CD117; PBT; SCFR

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer