KAP1 (TRIM28) Rabbit Polyclonal Antibody

CAT#: TA324442

Rabbit polyclonal TRIM28 Antibody (N-term)

Product Images

TRIM28 Antibody (N-term) (Cat. #TA324442) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).

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  TRIM28 Antibody (N-term) (Cat. #TA324442)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of Hela cell stained with TRIM28 Antibody (N-term) (Cat#[RB09760]).Hela cells were incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). TRIM28 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88550 Da
• Gene Name: tripartite motif containing 28
• Database Link:
  NP_005753
  Entrez Gene 10155 Human
  UniProt ID Q13263
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors