LEF1 Rabbit Polyclonal Antibody

CAT#: TA324467

Rabbit polyclonal LEF1 Antibody (N-term)

Product Images

LEF1 Antibody (N-term) (Cat. #TA324467) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LEF1 antibody detected the LEF1 protein (arrow).

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  IF image of A431 cell stained with LEF1 Antibody (N-term) (Cat#TA324467).A431 cells were incubated with LEF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). LEF1 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human LEF1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44201 Da
• Gene Name: lymphoid enhancer binding factor 1
• Database Link:
  NP_001124185
  Entrez Gene 16842 MouseEntrez Gene 161452 RatEntrez Gene 51176 Human
  UniProt ID Q9UJU2
• Synonyms: LEF-1; TCF1ALPHA; TCF7L3; TCF10

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway