KDM1A Rabbit Polyclonal Antibody

CAT#: TA324483

Rabbit polyclonal LSD1 Antibody (N-term)

Product Images

Western blot analysis of anti-LSD1 Pab (Cat. #TA324483) in mouse brain tissue lysate (35ug/lane). LSD1 (arrow) was detected using the purified Pab.

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  Western blot analysis of AOF2 (arrow) using LSD1 Antibody (N-term) (Cat.#TA324483).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of Hela cell stained with LSD1 Antibody (N-term) (Cat#TA324483).Hela cells were incubated with LSD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). LSD1 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, IF: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-142 amino acids from the N-terminal region of human LSD1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92903 Da
• Gene Name: lysine demethylase 1A
• Database Link:
  NP_001009999
  Entrez Gene 99982 MouseEntrez Gene 23028 Human
  UniProt ID O60341
• Synonyms: AOF2; BHC110; CPRF; KDM1; LSD1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors