Sumo 1 (SUMO1) Rabbit Polyclonal Antibody

CAT#: TA324522

Rabbit polyclonal SUMO1 Antibody (N-term)

Product Images

SUMO1 Antibody (N-term) (Cat. #TA324522) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SUMO1 antibody detected the SUMO1 protein (arrow).

• 

  Western blot analysis of lysate from mouse Neuro-2a cell line, using SUMO1 Antibody (N-term) (Cat. #TA324522). TA324522 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

• 

  SUMO1 Antibody (N-term) (Cat. #TA324522)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUMO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

• 

  IF image of A549 cell stained with SUMO1 Antibody (N-term) (Cat#TA324522).A549 cells were incubated with SUMO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).SUMO1 immunoreactivity is localized to Nuclear Membrane significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SUMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SUMO1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11557 Da
• Gene Name: small ubiquitin-like modifier 1
• Database Link:
  NP_001005781
  Entrez Gene 22218 MouseEntrez Gene 301442 RatEntrez Gene 7341 Human
  UniProt ID P63165
• Synonyms: DAP1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; UBL1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors