PITX2 Rabbit Polyclonal Antibody

CAT#: TA324544

Rabbit polyclonal PITX2 Antibody (C-term)

Product Images

Western blot analysis of anti-Pilx2 Pab (Cat.#TA324544) in CEM cell line lysates (35ug/lane). Pilx2 (arrow) was detected using the purified Pab.

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  IF image of Hela cell stained with PITX2 Antibody (C-term) (Cat#TA324544/SA070209B).Hela cells were incubated with PITX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).PITX2 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000
• Reactivities: Human (Predicted: Mouse, Rat, Zebrafish, Chicken, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the C-terminal region of human PITX2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35370 Da
• Gene Name: paired like homeodomain 2
• Database Link:
  NP_000316
  Entrez Gene 18741 MouseEntrez Gene 54284 RatEntrez Gene 5308 Human
  UniProt ID Q99697
• Synonyms: ARP1; Brx1; IDG2; IGDS; IGDS2; IHG2; IRID2; Otlx2; PTX2; RGS; RIEG; RIEG1; RS

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: TGF-beta signaling pathway