Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody

CAT#: TA324563

Rabbit polyclonal ALPL Antibody (Center)

Product Images

Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) (Cat.#TA324563). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.

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  ALPL Antibody (Center) (Cat.#TA324563) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).

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  ALPL Antibody (Center) (Cat. #TA324563)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of ALPL Antibody (Center) (Cat#TA324563) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  ALPL Antibody (Center) (Cat. #TA324563) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, FC: 1:10~50, IHC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ALPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the Central region of human ALPL.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57305 Da
• Gene Name: alkaline phosphatase, liver/bone/kidney
• Database Link:
  NP_000469
  Entrez Gene 11647 MouseEntrez Gene 249 Human
  UniProt ID P05186
• Synonyms: AP-TNAP; APTNAP; HOPS; TNAP; TNSALP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways