ECSIT Rabbit Polyclonal Antibody

CAT#: TA324573

Rabbit polyclonal ECSIT Antibody (C-term)

Product Images

ECSIT Antibody (C-term) (Cat. #TA324573) western blot analysis in Jurkat, ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).

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  IF image of Hela cell stained with ECSIT Antibody (C-term) (Cat#TA324573).Hela cells were incubated with ECSIT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ECSIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human ECSIT.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49148 Da
• Gene Name: ECSIT signalling integrator
• Database Link:
  NP_057665
  Entrez Gene 51295 Human
  UniProt ID Q9BQ95
• Synonyms: SITPEC

Reference Data

• Protein Pathways: MAPK signaling pathway