LIN28B Rabbit Polyclonal Antibody

CAT#: TA324574

Rabbit polyclonal LIN28B Antibody (N-term)

Product Images

Western blot analysis of LIN28B Antibody (N-term) (Cat.#TA324574) in HL60 cell line lysates (35ug/lane). LIN28B (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human Testis tissue reacted with LIN28B Antibody (N-term) (Cat.#TA324574), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of SY5Y cells stained with LIN28B (N-term) antibody. SY5Y cells were incubated with TA324574 LIN28B (N-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).

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  Flow cytometric analysis of HL-60 cells using LIN28B Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27084 Da
• Gene Name: lin-28 homolog B
• Database Link:
  NP_001004317
  Entrez Gene 389421 Human
  UniProt ID Q6ZN17
• Synonyms: CSDD2