SMAD9 Rabbit Polyclonal Antibody

CAT#: TA324579

Rabbit polyclonal SMAD9 Antibody (Center)

Product Images

SMAD9 Antibody (Center) (Cat. #TA324579) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).

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  IF image of MCF-7 cell stained with SMAD9 Antibody (Center) (Cat#TA324579). MCF-7 cells were incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52493 Da
• Gene Name: SMAD family member 9
• Database Link:
  NP_005896
  Entrez Gene 4093 Human
  UniProt ID O15198
• Synonyms: MADH6; MADH9; PPH2; SMAD8; SMAD8A; SMAD8B

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway