MAP1LC3A Rabbit Polyclonal Antibody

CAT#: TA324624

Rabbit polyclonal LC3 Antibody (APG8A) (N-term)

Product Images

Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #TA324624) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).

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  APG8a (MAP1LC3A) Antibody (M1) (Cat. #TA324624) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cells stained with TA324624 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine, then incubated with TA324624 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:200, IHC: 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14272 Da
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  NP_115903
  Entrez Gene 66734 MouseEntrez Gene 362245 RatEntrez Gene 84557 Human
  UniProt ID Q9H492
• Synonyms: ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3