ATG5 Rabbit Polyclonal Antibody

CAT#: TA324633

Rabbit polyclonal ATG5 Antibody (N-term)

Product Images

The anti-APG5L Pab (Cat. #TA324633) is used in Western blot to detect APG5L in mouse brain tissue lysate.

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  APG5L Antibody (D3) (Cat. #TA324633) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG5L antibody detected the APG5L protein (arrow).

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  Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3) (Cat. #TA324633). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

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  Western blot analysis of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3) (Cat. #TA324633). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autophagy APG5L antibody (N-term) (Cat.#TA324633), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

• IF image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine, then incubated with TA324633 ATG5 (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:200, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG5.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32447 Da
• Gene Name: autophagy related 5
• Database Link:
  NP_004840
  Entrez Gene 11793 MouseEntrez Gene 365601 RatEntrez Gene 9474 Human
  UniProt ID Q9H1Y0
• Synonyms: APG5; APG5-LIKE; APG5L; ASP; hAPG5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy, RIG-I-like receptor signaling pathway